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KMID : 0368420060490040291
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Journal of Plant Biology
2006 Volume.49 No. 4 p.291 ~ p.297
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Ethylene-regulated expression of ACC oxidase and ACC synthase genes in mung bean hypocotyls
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Kim Jeong-Hoe
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Abstract
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Ethylene induces an increase in transcript levels of the ACC oxidase gene (VR- ACO1) but suppresses expression of the ACC synthase gene (VR- ACS1) in etiolated mung bean hypocotyls. Here, treatment with either the transcription inhibitor α-amanitin or the protein synthesis inhibitor cycloheximide (CHI) completely abolished ethylene-induced accumulation ofVR- ACO1 mRNA. This indicated that ethylene-induction ofVR- ACO1 is due to transcriptional activation, which also relies onde novo protein synthesis. In contrast, CHI induced the accumulation ofVR- ACS1 transcripts. ABA also inhibited ethylene-inducedVR- ACO1 expression, but restored ethylene-suppressedVR- ACS1 expression. Results of time-course experiments and an interaction analysis of CHI and ABA suggested that the latter may exert its effect by preventing the synthesis of a factor(s) necessary for ethylene action. Ethylene-signaling was studied in more detail, using two pharmacological inhibitors ? EGTA and sodium orthovanadate. Those experiments demonstrated that calcium ions and a Tyr type of protein phosphatase may be involved in regulating ethylene biosynthetic genes.
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KEYWORD
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ABA, ACC oxidase, ACC synthase, calcium, ethylene, mung bean
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